Search results for "Alphavirus Infections"
showing 3 items of 3 documents
Transmission studies of a European Sindbis virus in the floodwater mosquito Aedes vexans (Diptera: Culicidae)
2002
Abstract Sindbis viruses are arthropod-borne viruses, which are maintained in nature in a Culex mosquitobird associated transmission cycle, but Aedes species have been suspected as playing a role in infecting humans. In this study, we addressed the question whether or not Germany's most abundant floodwater mosquito species Aedes vexans (Diptera, Culicidae) can serve as an efficient vector for Sindbis viruses. Firstly, the overall susceptibility of Ae. vexans was tested by intrathoracic inoculation of 40 plaque forming units (PFU) Karelian fever virus (KFV, an European Sindbis virus isolate) per female mosquito. Viral titres rose after inoculation reaching a maximum (about a 350-fold increas…
Development of a Sensitive Detection Method for Alphaviruses and Its Use as a Virus Neutralization Assay
2021
Alphaviruses have a single-stranded, positive-sense RNA genome that contains two open reading frames encoding either the non-structural or the structural genes. Upon infection, the genomic RNA is translated into the non-structural proteins (nsPs). NsPs are required for viral RNA replication and transcription driven from the subgenomic promoter (sgP). Transfection of an RNA encoding the luciferase gene under the control of the sgP into cells enabled the detection of replication-competent chikungunya virus (CHIKV) or Mayaro virus (MAYV) with high sensitivity as a function of the induced luciferase activity. This assay principle was additionally used to analyze virus-neutralizing antibodies in…
No influence of oxygen levels on pathogenesis and virus shedding in Salmonid alphavirus (SAV)-challenged Atlantic salmon (Salmo salar L.)
2010
Abstract Background For more than three decades, diseases caused by salmonid alphaviruses (SAV) have become a major problem of increasing economic importance in the European fish-farming industry. However, experimental infection trials with SAV result in low or no mortality i.e very different from most field outbreaks of pancreas disease (PD). This probably reflects the difficulties in reproducing complex biotic and abiotic field conditions in the laboratory. In this study we looked at the relationship between SAV-infection in salmon and sub-lethal environmental hypoxia as a result of reduced flow-through in tank systems. Results The experiment demonstrated that constant reduced oxygen leve…